Yeast vacuoles and membrane fusion pathways
نویسندگان
چکیده
منابع مشابه
In vitro assay using engineered yeast vacuoles for neuronal SNARE-mediated membrane fusion.
Intracellular membrane fusion requires not only SNARE proteins but also other regulatory proteins such as the Rab and Sec1/Munc18 (SM) family proteins. Although neuronal SNARE proteins alone can drive the fusion between synthetic liposomes, it remains unclear whether they are also sufficient to induce the fusion of biological membranes. Here, through the use of engineered yeast vacuoles bearing...
متن کاملFusion of SV40-induced endocytotic vacuoles with the nuclear membrane.
The interaction between simian virus 40(SV40)-induced endocytotic vacuoles and the nuclear membrane was investigated using cationized ferritin (CF) and concanavalin A (Con A) as cell membrane markers. These markers bound to the cell surfaces of CV-1 cells together with SV40 at 4 degrees C. Following incubation of these modified cells at 37 degrees C in serum-free medium, the cell membranes show...
متن کاملSec18p (NSF)-Driven Release of Sec17p (α-SNAP) Can Precede Docking and Fusion of Yeast Vacuoles
and the plasma membrane in epithelial cells requires S.cerevisiae inherits its vacuole by projecting vacuoleNSF for the basolateral route, but probably not for the derived membrane vesicles and tubules into the bud, apical route (Ikonen et al., 1995). Homotypic ER to ER where they fuse to establish the daughter vacuole. fusion and nuclear fusion during yeast mating depend on This homotypic fusi...
متن کاملSec18p (NSF)-driven release of Sec17p (alpha-SNAP) can precede docking and fusion of yeast vacuoles.
S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic fusion event can be assayed in vitro. It requires Sec17p and Sec18p, the homologs of the mammalian alpha-SNAP and NSF, which cooperate in multiple steps of membrane trafficking. We now report that Sec17p, Sec18p, and ATP a...
متن کاملTrans-SNARE complex assembly and yeast vacuole membrane fusion.
cis-SNARE complexes (anchored in one membrane) are disassembled by Sec17p (alpha-SNAP) and Sec18p (NSF), permitting the unpaired SNAREs to assemble in trans. We now report a direct assay of trans-SNARE complex formation during yeast vacuole docking. SNARE complex assembly and fusion is promoted by high concentrations of the SNARE Vam7p or Nyv1p or by addition of HOPS (homotypic fusion and vacuo...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2002
ISSN: 0261-4189
DOI: 10.1093/emboj/21.6.1241